Generator

Part:BBa_K1053006:Design

Designed by: Ippei Sakamoto   Group: iGEM13_Tokyo-NoKoGen   (2013-09-27)

PBAD-TR(42)-HHR-taR12-DT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1338
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1145
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1338
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1338
    Illegal AgeI site found at 980
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 962


Design Notes

This hammerhead ribozyme(HHR) has trans-acting RNA(taRNA) responsive region, and this HHR responses to taR42. In absence of taR42, the HHR can self-cleave, but in presence of taR42, their capability of self-cleavage is inhibited due to hybridize with taR42 and change their structures. This HHR has taR12 in the downstream. taR12 and taR42 are orthogonal riboregulators reported by Callura et al.

Source

BBa_K1053011 & BBa_K1053008
Overlap PCR was performed to connect BBa_K1053011 and taR12-DT in BBa_K1053008.

References

Callura,J.M., Cantor,C.R. and Collins,J.J. (2012) Genetic switchboard for synthetic biology applications. Proc. Natl Acad. Sci. USA, 109, 5850–5855.